MedwayOne is located approximately three miles east of the community of Hoo St Werburgh and approximately nine miles north east of the town of Chatham.

It is nine miles to Junction 1 of the M2 motorway, and just 18 miles from Junction 2 of the M25 motorway. The southern and eastern boundaries of the site are met by the mouth of the Medway River estuary, where there is a flood defence system in place. To the west of the site are a number of agricultural fields.